A Gene-Expression Screen Identifies A Non-Toxic Sumoylation
نویسندگان
چکیده
Department of Cellular and Molecular Pharmacology, 9 Small Molecule Discovery Center, Department of Pharmaceutical Chemistry, 10 Department of Biochemistry and Biophysics 11 Department of Epidemiology and Biostatistics, 12 University of California, San Francisco, 13 San Francisco, California 94158, USA 14 Chemical Biology Laboratory 15 National Cancer Institute 16 376 Boyles Street 17 Frederick, MD 21702, USA 18 These authors contributed equally 19
منابع مشابه
A gene-expression screen identifies a non-toxic sumoylation inhibitor that mimics SUMO-less human LRH-1 in liver.
SUMO-modification of nuclear proteins has profound effects on gene expression. However, non-toxic chemical tools that modulate sumoylation in cells are lacking. Here, to identify small molecule sumoylation inhibitors we developed a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor Homolog-1 (hLRH-1, NR5A2). Our primary gene-expression screen assayed two SUMO-...
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